rabbit polyclonal anti-fli1 antibody  (Santa Cruz Biotechnology)

Journal: Journal of Inflammation Research

Article Title: FLI1 Regulates Histamine Decarboxylase Expression to Control Inflammation Signaling and Leukemia Progression

doi: 10.2147/JIR.S401566

Figure Lengend Snippet: The Primer Sequences are Listed

Article Snippet: Western blotting was done using protocol, as previously described., The antibodies used are as follows: Polyclonal rabbit antibodies for FLI1 (Cat. no. ab133485) and HDC (Cat. no. ab137571) were obtained from Abcam, the monoclonal GAPDH (Cat. no. G9545) antibody was obtained from Sigma Aldrich; goat anti mouse and goat anti rabbit HRP conjugated antibodies were obtained from Cell Signaling Technology (Cat. nos.

Techniques:

Journal: Journal of Inflammation Research

Article Title: FLI1 Regulates Histamine Decarboxylase Expression to Control Inflammation Signaling and Leukemia Progression

doi: 10.2147/JIR.S401566

Figure Lengend Snippet: The HDC expression is regulated by FLI1. ( A and B ) ShFLI1 and control cells were subjected to RT-qPCR analysis for the expression of FLI1 ( A ) and HDC ( B ) genes. ( C ) ShFLI1 and scrambled control cells were examined for expression of FLI1 and HDC using Western blotting. Rd: Relative density determined using densitometer. ( D ) Relative expression of FLI1 protein in inducible K562-fli-1 cells after addition of Doxycycline. ( E ) Relative expression of HDC by RT-qPCR in K562-fli-1 cells after addition of doxycycline. ( F ) Chromatin immunoprecipitation (ChIp) analysis of the HDC promoter in HEL cells for binding to FLI1, by RT-qPCR (Lower panel). Upper panel represents the gel image of the immunoprecipitated PCR-amplified band relative to input. P=<0.01 (**), P=<0.001 (***) and P=<0.0001 (****) by two-tailed Student’s t-test.

Article Snippet: Western blotting was done using protocol, as previously described., The antibodies used are as follows: Polyclonal rabbit antibodies for FLI1 (Cat. no. ab133485) and HDC (Cat. no. ab137571) were obtained from Abcam, the monoclonal GAPDH (Cat. no. G9545) antibody was obtained from Sigma Aldrich; goat anti mouse and goat anti rabbit HRP conjugated antibodies were obtained from Cell Signaling Technology (Cat. nos.

Techniques: Expressing, Control, Quantitative RT-PCR, Western Blot, Chromatin Immunoprecipitation, Binding Assay, Immunoprecipitation, Amplification, Two Tailed Test

Journal: Journal of Inflammation Research

Article Title: FLI1 Regulates Histamine Decarboxylase Expression to Control Inflammation Signaling and Leukemia Progression

doi: 10.2147/JIR.S401566

Figure Lengend Snippet: Epigallocatechin (EGC) suppresses HDC transcriptional expression in HEL cells independent of FLI1. ( A ) Chemical structure of EGC. ( B ) Expression of HDC in HEL cells treated with the indicated concentration of EGC, as determined by RT-qPCR. ( C ) EGC marginally affect cell proliferation of HEL cells in culture. ( D ) HDC expression does not affect the expression of FLI1, as determine by Western blot. ( E ) Heatmap of expression of the indicated genes in shFLI1 versus control cells (Left panel). The relative expression level was determined by RNAseq analysis, as shown in the right panel. P=<0.001 (***) by two-tailed Student’s t-test.

Article Snippet: Western blotting was done using protocol, as previously described., The antibodies used are as follows: Polyclonal rabbit antibodies for FLI1 (Cat. no. ab133485) and HDC (Cat. no. ab137571) were obtained from Abcam, the monoclonal GAPDH (Cat. no. G9545) antibody was obtained from Sigma Aldrich; goat anti mouse and goat anti rabbit HRP conjugated antibodies were obtained from Cell Signaling Technology (Cat. nos.

Techniques: Expressing, Concentration Assay, Quantitative RT-PCR, Western Blot, Control, Two Tailed Test

Journal: Journal of Inflammation Research

Article Title: FLI1 Regulates Histamine Decarboxylase Expression to Control Inflammation Signaling and Leukemia Progression

doi: 10.2147/JIR.S401566

Figure Lengend Snippet: FLI1 regulates genes associated with the asthmatic reaction. ( A – C ) Relative expression of the CPA3 ( A ), IL1B ( B ) and CXCR2 ( C ) genes in shFLI1 versus control cells, by RT-qPCR. ( D – F ). Relative expression of the CPA3 ( D ), IL1B ( E ) and CXCR2 ( F ) genes in shHDC2-3 cells, by RT-qPCR. ( G ) Relative expression of FLI1 in shHDC1-3 cells, by Western blot. P=<0.05 (*), P=<0.01 (**) and P=<0.001 (***) by two-tailed Student’s t-test.

Article Snippet: Western blotting was done using protocol, as previously described., The antibodies used are as follows: Polyclonal rabbit antibodies for FLI1 (Cat. no. ab133485) and HDC (Cat. no. ab137571) were obtained from Abcam, the monoclonal GAPDH (Cat. no. G9545) antibody was obtained from Sigma Aldrich; goat anti mouse and goat anti rabbit HRP conjugated antibodies were obtained from Cell Signaling Technology (Cat. nos.

Techniques: Expressing, Control, Quantitative RT-PCR, Western Blot, Two Tailed Test

Journal: Journal of Inflammation Research

Article Title: FLI1 Regulates Histamine Decarboxylase Expression to Control Inflammation Signaling and Leukemia Progression

doi: 10.2147/JIR.S401566

Figure Lengend Snippet: Tetrandrine inhibits HDC through downregulation of FLI1. ( A ) Chemical structure of tetrandrine. ( B and C ) Tetrandrine inhibits FLI1 protein ( B ) and its transcript ( C ) in HEL cells in a dose dependent manner. ( D ) Tetrandrine inhibit HDC in a dose dependent manner, as determined by RT-qPCR. ( E ) Tetrandrine activated the expression of miR145 in a dose dependent manner. ( F ) Tetrandrine inhibits HEL cell proliferation in culture. ( G ) Tetrandrine induces cell death of HEL cells in a dose dependent manner. ( G ) Cells were photographed 24 h post-drug treatment (−50µm; magnification 20X). Black arrows shows death cells. P=<0.05 (*), P=<0.01 (**) and P=<0.001 (***) by two-tailed Student’s t-test.

Article Snippet: Western blotting was done using protocol, as previously described., The antibodies used are as follows: Polyclonal rabbit antibodies for FLI1 (Cat. no. ab133485) and HDC (Cat. no. ab137571) were obtained from Abcam, the monoclonal GAPDH (Cat. no. G9545) antibody was obtained from Sigma Aldrich; goat anti mouse and goat anti rabbit HRP conjugated antibodies were obtained from Cell Signaling Technology (Cat. nos.

Techniques: Quantitative RT-PCR, Expressing, Two Tailed Test

Journal: Journal of Inflammation Research

Article Title: FLI1 Regulates Histamine Decarboxylase Expression to Control Inflammation Signaling and Leukemia Progression

doi: 10.2147/JIR.S401566

Figure Lengend Snippet: Diacerein and tetrandrine inhibit leukemia progression in vivo. ( A – D ) Leukemic mice induced by F-MuLV were treated with 5 mg/kg histamine ( A ), 3 mg/kg famotidine ( B ), 3 mg/Kg diacerein ( C ) and 3 mg/Kg tetrandrine ( D ) for two weeks. Mice were observed for the development of leukemia and scored at the onset of death. The tumor growth was calculated by Student’s t-test and death was used to plot a Kaplan–Meier survival curve. ( E ) In this model, FLI1 controls HDC transcription, which itself regulates histamine production involved in allergenic reaction. HDC is also controls CXCR2 and IL1B associated with asthmatic reaction. These inflammatory factors likely effects leukemia progression through inflammation. While tetrandrine inhibits HDC through FLI1, EGC controls transcription of HDC through another mechanism.

Article Snippet: Western blotting was done using protocol, as previously described., The antibodies used are as follows: Polyclonal rabbit antibodies for FLI1 (Cat. no. ab133485) and HDC (Cat. no. ab137571) were obtained from Abcam, the monoclonal GAPDH (Cat. no. G9545) antibody was obtained from Sigma Aldrich; goat anti mouse and goat anti rabbit HRP conjugated antibodies were obtained from Cell Signaling Technology (Cat. nos.

Techniques: In Vivo